Nanoscopic Characterization of Cell Migration under Flow Using Optical and Electron Microscopy.

ABSTRACT: Hematopoietic stem/progenitor cell (HSPC) and leukemic cell homing is an important biological phenomenon that takes place through essential interactions with adhesion molecules on an endothelial cell layer. The homing process of HSPCs begins with the tethering and rolling of the cells on the endothelial layer, which is achieved by the interaction between selectins on the endothelium to the ligands on HSPC/leukemic cells under shear stress of the blood flow. Although many studies have been based on in vitro conditions of the cells rolling over recombinant proteins, significant challenges remain when imaging HSPC/leukemic cells on the endothelium, a necessity when considering characterizing cell-to-cell interaction and rolling dynamics during cell migration. Here, we report a new methodology

SUBMITTER: Alghamdi A 

PROVIDER: S-EPMC9878504 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature