A Pipeline for the Development of Microsatellite Markers using Next Generation Sequencing Data.

ABSTRACT: Background: Also known as Simple Sequence Repetitions (SSRs), microsatellites are profoundly informative molecular markers and powerful tools in genetics and ecology studies on plants. Objective: This research presents a workflow for developing microsatellite markers using genome skimming. Methods: The pipeline was proposed in several stages that must be performed sequentially: obtaining DNA sequences, identifying microsatellite regions, designing primers, and selecting candidate microsatellite regions to develop the markers. Our pipeline efficiency was analyzed using Illumina sequencing data from the non-model tree species Pterodon emarginatus Vog. Results: The pipeline revealed 4,382 microsatellite regions and drew 7,411 pair

SUBMITTER: Antunes AM 

PROVIDER: S-EPMC9878831 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature