Auxin-inducible degron 2 system deciphers functions of CTCF domains in transcriptional regulation.

ABSTRACT:

Background

CTCF is a well-established chromatin architectural protein that also plays various roles in transcriptional regulation. While CTCF biology has been extensively studied, how the domains of CTCF function to regulate transcription remains unknown. Additionally, the original auxin-inducible degron 1 (AID1) system has limitations in investigating the function of CTCF.

Results

We employ an improved auxin-inducible degron technology, AID2, to facilitate the study of acute depletion of CTCF while overcoming the limitations of the previous AID system. As previously observed through the AID1 system and steady-state RNA analysis, the new AID2 system combined with SLAM-seq confirms that CTCF depletion leads to modest nascent and steady-state transcript changes. A CTCF domain

SUBMITTER: Hyle J 

PROVIDER: S-EPMC9878928 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature