Particle Localization Using Local Gradients and Its Application to Nanometer Stabilization of a Microscope.

ABSTRACT: Particle localization plays a fundamental role in advanced biological techniques such as single-molecule tracking, superresolution microscopy, and manipulation by optical and magnetic tweezers. Such techniques require fast and accurate particle localization algorithms as well as nanometer-scale stability of the microscope. Here, we present a universal method for three-dimensional localization of single labeled and unlabeled particles based on local gradient calculation of particle images. The method outperforms state-of-the-art localization techniques in high-noise conditions, and it is capable of 3D nanometer accuracy localization of nano- and microparticles with sub-millisecond calculation time. By localizing a fixed particle as fiducial mark and running a feedback loop, we demonstrate i

SUBMITTER: Kashchuk AV 

PROVIDER: S-EPMC9878972 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature