Expansion Microscopy for Imaging the Cell-Material Interface.

ABSTRACT: Surface topography on the scale of tens of nanometers to several micrometers substantially affects cell adhesion, migration, and differentiation. Recent studies using electron microscopy and super-resolution microscopy provide insight into how cells interact with surface nanotopography; however, the complex sample preparation and expensive imaging equipment required for these methods makes them not easily accessible. Expansion microscopy (ExM) is an affordable approach to image beyond the diffraction limit, but ExM cannot be readily applied to image the cell-material interface as most materials do not expand. Here, we develop a protocol that allows the use of ExM to resolve the cell-material interface with high resolution. We apply the technique to image the interface between U2OS cells an

SUBMITTER: Nakamoto ML 

PROVIDER: S-EPMC9879138 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature