Assessing the breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring.

ABSTRACT:

Background

Climate change has driven shifts in breeding phenology of many amphibians, causing phenological mismatches (e.g., predator-prey interactions), and potentially population declines. Collecting data with high spatiotemporal sensitivity on hibernation emergence and breeding times can inform conservation best practices. However, monitoring the phenology of amphibians can be challenging because of their cryptic nature over much of their life cycle. Moreover, most salamanders and caecilians do not produce conspicuous breeding calls like frogs and toads do, presenting additional monitoring challenges.

Methods

In this study, we designed and evaluated the performance of an environmental DNA (eDNA) droplet digital PCR (ddPCR) assay as a non-invasive tool to assess the

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9879156 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature