Shuffling the yeast genome using CRISPR/Cas9-generated DSBs that target the transposable Ty1 elements.

ABSTRACT: Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified. In the genome of the yeast Saccharomyces cerevisiae, the most common class of transposon is the retrotransposon Ty1. Here, we explored how Cas9-induced double-strand breaks (DSBs) directed to Ty1 elements produce genomic alterations in this yeast species. Following Cas9 induction, we observed a significant elevation of chromosome rearrangements such as deletions, duplications and translocations. In addition, we found elevated rates of mitotic recombination, resulting in loss of heterozygosity. Using Southern analysis coupled with short- and long-read DNA sequencing, we revealed impo

SUBMITTER: Qi L 

PROVIDER: S-EPMC9879454 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature