Transmission structured illumination microscopy with tunable frequency illumination using tilt mirror assembly.

ABSTRACT: We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the illumination module where the sample is excited with the interference of two beams reflected from the opposite pair of mirror facets. Tunable frequency structured patterns are generated by changing the mirror-tilt angle and the hexagonal-symmetric arrangement is considered for the isotropic resolution in three orientations. Utilizing high numerical aperture (NA) objective in standard SIM provides super-resolution compromising with the field-of-view (FOV). Employing low NA (20X/0.4) objective lens detection, we experimentally demonstrate [Formula: see te

SUBMITTER: Samanta K 

PROVIDER: S-EPMC9879979 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature