Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN.

ABSTRACT: Just one amino acid at the carboxy-terminus of the B chain distinguishes human insulin from porcine insulin. By introducing a precise point mutation into the porcine insulin (INS) gene, we were able to generate genetically modified pigs that secreted human insulin; these pigs may be suitable donors for islet xenotransplantation. The electroporation of the CRISPR/Cas9 gene-editing system into zygotes is frequently used to establish genetically modified rodents, as it requires less time and no micromanipulation. However, electroporation has not been used to generate point-mutated pigs yet. In the present study, we introduced a point mutation into porcine zygotes via electroporation using the CRISPR/Cas9 system to generate INS point-mutated pigs as suitable islet d

SUBMITTER: Tanihara F 

PROVIDER: S-EPMC9880039 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature