Novel, alternative splicing signature to detect lymph node metastasis in prostate adenocarcinoma with machine learning.

ABSTRACT:

Background

The presence of lymph node metastasis leads to a poor prognosis for prostate cancer (Pca). Recently, many studies have indicated that gene signatures may be able to predict the status of lymph nodes. The purpose of this study is to probe and validate a new tool to predict lymph node metastasis (LNM) based on alternative splicing (AS).

Methods

Gene expression profiles and clinical information of prostate adenocarcinoma cohort were retrieved from The Cancer Genome Atlas (TCGA) database, and the corresponding RNA-seq splicing events profiles were obtained from the TCGA SpliceSeq. Limma package was used to identify the differentially expressed alternative splicing (DEAS) events between LNM and non-LNM groups. Eight machine learning classifiers were built to train with

SUBMITTER: Xie P 

PROVIDER: S-EPMC9880415 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature