Evaluation of a vaccine candidate isolated from Cryptosporidium parvum oocyst in mice.

ABSTRACT:

Background and aim

Cryptosporidiosis is a leading cause of diarrheal disease worldwide and is an animal and public health burden. This study aimed to evaluate the protective potential of affinity-purified Cryptosporidium parvum oocyst antigen as a vaccine candidate according to fecal oocyst shedding, humoral and cellular immune responses, histopathological changes, and the number of parasite developmental stages in ileal and hepatic tissues.

Materials and methods

We isolated oocysts from naturally infected buffalo calves and identified them molecularly as C. parvum isolates (GenBank: ON730707 and ON730708) by targeting the Cryptosporidium oocyst wall protein gene. We propagated the C. parvum oocysts in mice. In addition, we prepared crude antigen

SUBMITTER: Aboelsoued D 

PROVIDER: S-EPMC9880841 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature