Molecular characterization of Newcastle disease virus vaccines in Nigeria.

ABSTRACT:

Background and aim

Newcastle disease (ND) caused by ND virus (NDV) is a serious impediment to effective poultry production in developing countries such as Nigeria. Despite employing vaccination and other control measures to curtail this disease, its severe forms still persist. This study aimed to confirm the virus strains in the NDV vaccine brands commonly used in Nigeria.

Materials and methods

We employed reverse-transcription polymerase chain reaction (RT-PCR), sequencing, and sequence analysis to characterize NDV strains in four NDV vaccines commonly used in Nigeria. Fragments of 300 bp from NDV fusion genes from the vaccines were amplified. Polymerase chain reaction products were sequenced and analyzed using multiple sequence alignment and phylogenetic analyses to charac

SUBMITTER: Sajo MU 

PROVIDER: S-EPMC9880846 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature