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Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy.

ABSTRACT: We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells, and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of photoactivatable labels for strain-promoted tetrazine ligation and self-labeling protein tags (HaloTag, SNAP-tag), and duplexing of an imaging channel with another large Stokes shift dye have been demonstrated.

SUBMITTER: Likhotkin I

PROVIDER: S-EPMC9880998 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy.

Likhotkin Ilya I Lincoln Richard R Bossi Mariano L ML Butkevich Alexey N AN Hell Stefan W SW

Journal of the American Chemical Society 20230110 3

We designed caging-group-free photoactivatable live-cell permeant dyes with red fluorescence emission and ∼100 nm Stokes shifts based on a 1-vinyl-10-silaxanthone imine core structure. The proposed fluorophores undergo byproduct-free one- and two-photon activation, are suitable for multicolor fluorescence microscopy in fixed and living cells, and are compatible with super-resolution techniques such as STED (stimulated emission depletion) and PALM (photoactivated localization microscopy). Use of ...[more]

PMID: 36626161

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