The contribution of the alternative pathway in complement activation on cell surfaces depends on the strength of classical pathway initiation.

ABSTRACT:

Objectives

The complement system is an important component of innate immunity. The alternative pathway (AP) amplification loop is considered an essential feed forward mechanism for complement activation. However, the role of the AP in classical pathway (CP) activation has only been studied in ELISA settings. Here, we investigated its contribution on physiologically relevant surfaces of human cells and bacterial pathogens and in antibody-mediated complement activation, including in autoimmune haemolytic anaemia (AIHA) setting with autoantibodies against red blood cells (RBCs).

Methods

We evaluated the contribution of the AP to complement responses initiated through the CP on human RBCs by serum of AIHA patients and recombinant antibodies. Moreover, we studied complement activ

SUBMITTER: de Boer EC 

PROVIDER: S-EPMC9881211 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature