RNA-seq preprocessing and sample size considerations for gene network inference.

ABSTRACT:

Background

Gene network inference (GNI) methods have the potential to reveal functional relationships between different genes and their products. Most GNI algorithms have been developed for microarray gene expression datasets and their application to RNA-seq data is relatively recent. As the characteristics of RNA-seq data are different from microarray data, it is an unanswered question what preprocessing methods for RNA-seq data should be applied prior to GNI to attain optimal performance, or what the required sample size for RNA-seq data is to obtain reliable GNI estimates.

Results

We ran 9144 analysis of 7 different RNA-seq datasets to evaluate 300 different preprocessing combinations that include data transformations, normalizations and association estimators. We found t

SUBMITTER: Altay G 

PROVIDER: S-EPMC9881880 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature