Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen <i>B. burgdorferi</i>.

ABSTRACT: Transcription termination is an essential and dynamic process that can tune gene expression in response to diverse molecular signals. Yet, the genomic positions, molecular mechanisms, and regulatory consequences of termination have only been studied thoroughly in model bacteria. We employed complementary RNA-seq approaches to map RNA ends for the transcriptome of the spirochete Borrelia burgdorferi - the etiological agent of Lyme disease. By systematically mapping B. burgdorferi RNA ends at single nucleotide resolution, we delineated complex gene arrangements and operons and mapped untranslated regions (UTRs) and small RNAs (sRNAs). We experimentally tested modes of B. burgdorferi transcription termination and compared our findings to observations in E. coli , <

SUBMITTER: Petroni E 

PROVIDER: S-EPMC9881889 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature