At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring.

ABSTRACT: As the field of synthetic biology expands, the need to grow and train science, technology, engineering, and math (STEM) practitioners is essential. However, the lack of access to hands-on demonstrations has led to inequalities of opportunity and practice. In addition, there is a gap in providing content that enables students to make their own bioengineered systems. To address these challenges, we develop four shelf-stable cell-free biosensing educational modules that work by just-adding-water and DNA to freeze-dried crude extracts of Escherichia coli . We introduce activities and supporting curricula to teach the structure and function of the lac operon, dose-responsive behavior, considerations for biosensor outputs, and a 'build-your-own' activity for monitoring environmenta

SUBMITTER: Jung KJ 

PROVIDER: S-EPMC9881948 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature