High-parameter phenotypic characterization reveals a subset of human Th17 cells that preferentially produce IL17 against M. tuberculosis antigen.

ABSTRACT:

Background

Interleukin 17 producing CD4 T cells contribute to the control of Mycobacterium tuberculosis (Mtb) infection in humans; whether infection with Human Immunodeficiency Virus (HIV) disproportionately affects distinct Th17 cell subsets that respond to Mtb is incompletely defined.

Methods

We performed high-definition characterization of circulating Mtb-specific Th17 cells by spectral flow cytometry in people with latent TB and treated HIV (HIV-ART). We also measured kynurenine pathway activity by LC/MS on plasma and tested the hypothesis that tryptophan catabolism influences Th17 cell frequencies in this context.

Results

We identified two subsets of Th17 cells: subset 1 defined as CD4⁺Vα7.2^-CD161⁺CD26^+</

SUBMITTER: Ogongo P 

PROVIDER: S-EPMC9881994 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature