Specificity Guides Interpretation: On H3K4 Methylation at Enhancers and Broad Promoters.

ABSTRACT: In 2018, we used internally calibrated chromatin immunoprecipitation (ICeChIP) to find that many of the most commonly used antibodies against H3K4 methylforms had significant off-target binding, which compromised the findings of at least eight literature paradigms that used these antibodies for ChIP-seq (Shah et al., 2018). In many cases, we were able to recapitulate the prior findings in K562 cells with the original, low-quality antibody, only to find that the models did not hold up to scrutiny with highly specific reagents and quantitative calibration. In a recent preprint originally prepared as a Letter to the Editor of Molecular Cell, though they agree with our overarching conclusions, Pekowska and colleagues take issue with analyses presented for two relatively minor points of the pap

SUBMITTER: Shah RN 

PROVIDER: S-EPMC9882130 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature