Ribosome rescue factor PELOTA modulates translation start site choice and protein isoform levels of transcription factor C/EBPα.

ABSTRACT: Translation initiation at alternative start sites can dynamically control the synthesis of two or more functionally distinct protein isoforms from a single mRNA. Alternate isoforms of the hematopoietic transcription factor CCAAT-enhancer binding protein α (C/EBPα) produced from different start sites exert opposing effects during myeloid cell development. This alternative initiation depends on sequence features of the CEBPA transcript, including a regulatory upstream open reading frame (uORF), but the molecular basis is not fully understood. Here we identify trans-acting factors that affect C/EBPα isoform choice using a sensitive and quantitative two-color fluorescence reporter coupled with CRISPRi screening. Our screen uncovered a role for the ribosome re

SUBMITTER: Fernandez SG 

PROVIDER: S-EPMC9882168 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature