Sex-dependent Differences in the Genomic Profile of Lingual Sensory Neurons in Naive and Tongue-Tumor Bearing Mice.

ABSTRACT: Mechanisms of sex-dependent orofacial pain are widely understudied. A significant gap in knowledge exists about comprehensive regulation of tissue-specific trigeminal sensory neurons in diseased state of both sexes. Using RNA sequencing of FACS sorted retro-labeled sensory neurons innervating tongue tissue, we determined changes in transcriptomic profiles in males and female mice under naïve as well as tongue-tumor bearing conditions Our data revealed the following interesting findings: 1) Tongue tissue of female mice was innervated with higher number of trigeminal neurons compared to males; 2) Naïve female neurons innervating the tongue exclusively expressed immune cell markers such as Csf1R, C1qa and others, that weren't expressed in males. This was validated by Immunohistochemistry. 4)

SUBMITTER: Ibrahim T 

PROVIDER: S-EPMC9882171 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature