Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein.

ABSTRACT: Cytoplasmic dynein, the primary retrograde microtubule transport motor within cells, must be activated for processive motility through the regulated assembly of a dynein-dynactin-adapter (DDA) complex. The interaction between dynein and dynactin was initially ascribed to the N-terminus of the dynein intermediate chain (IC) and a coiled-coil of the dynactin subunit p150 ^Glued . However, cryo-EM structures of DDA complexes have not resolve these regions of the IC and p150 ^Glued , raising questions about the importance of this interaction. The IC N-terminus (ICN) also interacts with the dynein regulators Nde1/Ndel1, which compete with p150 ^Glued for binding to ICN. Using a combination of approaches, we reveal that the ICN plays critical, evolutionarily conserv

SUBMITTER: Okada K 

PROVIDER: S-EPMC9882231 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature