Stochastic motion and transcriptional dynamics of pairs of distal DNA loci on a compacted chromosome.

ABSTRACT: Chromosomes in the eukaryotic nucleus are highly compacted. However, for many functional processes, including transcription initiation, the 3D pair-wise motion of distal chromosomal elements, such as enhancers and promoters, is essential and necessitates dynamic fluidity. Therefore, the interplay of chromosome organization and dynamics is crucial for gene regulation. Here, we use a live imaging assay to simultaneously measure the positions of pairs of enhancers and promoters and their transcriptional output in the developing fly embryo while systematically varying the genomic separation between these two DNA loci. Our analysis reveals a combination of a compact globular organization and fast subdiffusive dynamics. These combined features cause an anomalous scaling of polymer relaxation tim

SUBMITTER: Bruckner DB 

PROVIDER: S-EPMC9882297 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature