Molecular Logic of Synaptic Diversity Between Drosophila Tonic and Phasic Motoneurons.

ABSTRACT: Although neuronal subtypes display unique synaptic organization and function, the underlying transcriptional differences that establish these features is poorly understood. To identify molecular pathways that contribute to synaptic diversity, single neuron PatchSeq RNA profiling was performed on Drosophila tonic and phasic glutamatergic motoneurons. Tonic motoneurons form weaker facilitating synapses onto single muscles, while phasic motoneurons form stronger depressing synapses onto multiple muscles. Super-resolution microscopy and in vivo imaging demonstrated synaptic active zones in phasic motoneurons are more compact and display enhanced Ca ²⁺ influx compared to their tonic counterparts. Genetic analysis identified unique synaptic properties that mapped onto ge

SUBMITTER: Jetti SK 

PROVIDER: S-EPMC9882338 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature