Blind Assessment of Monomeric AlphaFold2 Protein Structure Models with Experimental NMR Data.

ABSTRACT: Recent advances in molecular modeling of protein structures are changing the field of structural biology. AlphaFold-2 (AF2), an AI system developed by DeepMind, Inc., utilizes attention-based deep learning to predict models of protein structures with high accuracy relative to structures determined by X-ray crystallography and cryo-electron microscopy (cryoEM). Comparing AF2 models to structures determined using solution NMR data, both high similarities and distinct differences have been observed. Since AF2 was trained on X-ray crystal and cryoEM structures, we assessed how accurately AF2 can model small, monomeric, solution protein NMR structures which (i) were not used in the AF2 training data set, and (ii) did not have homologous structures in the Protein Data Bank at the time of

SUBMITTER: Li EH 

PROVIDER: S-EPMC9882346 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature