Computational Analysis of Maize Enhancer Regulatory Elements Using ATAC-STARR-seq.

ABSTRACT: The blueprints to development, response to the environment, and cellular function are largely the manifestation of distinct gene expression programs controlled by the spatiotemporal activity of cis-regulatory elements. Although biochemical methods for identifying accessible chromatin - a hallmark of active cis-regulatory elements - have been developed, approaches capable of measuring and quantifying cis-regulatory activity are only beginning to be realized. Massively Parallel Reporter Assays coupled to chromatin accessibility profiling present a high-throughput solution for testing the transcription-activating capacity of millions of putatively regulatory DNA sequences in parallel. However, clear computational pipelines for analyzing these high-throughput sequencing-ba

SUBMITTER: Marand AP 

PROVIDER: S-EPMC9882361 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature