Netrin-1 binding to Unc5B regulates Blood-Retina Barrier integrity.

ABSTRACT:

Background

The blood brain barrier (BBB) preserves neuronal function in the central nervous system (CNS) by tightly controlling metabolite exchanges with the blood. In the eye, the retina is likewise protected by the blood-retina barrier (BRB) to maintain phototransduction. We showed that the secreted guidance cue Netrin-1 regulated BBB integrity, by binding to endothelial Unc5B and regulating canonical β-catenin dependent expression of BBB gene expression.

Objective

Here, we investigated if Netrin-1-binding to endothelial Unc5B also controlled BRB integrity, and if this process involved Norrin/β-catenin signaling, which is the major known driver of BRB development and maintenance.

Methods

We analyzed Tamoxifen-inducible loss- and gain- of-function alleles of Unc5B

SUBMITTER: Furtado J 

PROVIDER: S-EPMC9882365 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature