Elucidation of the Molecular Basis and Cellular Functions of Vinculin-Actin Directional Catch Bonding.

ABSTRACT: The ability of cells and tissues to differentially resist or adapt to mechanical forces applied in distinct directions is mediated by the ability of load-bearing proteins to preferentially maintain physical linkages in certain directions. However, the molecular basis and biological consequences of directional force-sensitive binding are unclear. Vinculin (Vcn) is a load-bearing linker protein that exhibits directional catch bonding due to interactions between the Vcn tail domain (Vt) and filamentous (F)-actin. We developed a computational approach to predict Vcn residues involved in directional catch bonding and produced a set of associated Vcn variants with unaltered Vt structure, actin binding, or phospholipid interactions. Incorporation of these variants into Vcn biosensors did not pert

SUBMITTER: Chirasani VR 

PROVIDER: S-EPMC9882595 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature