Combinatorial quantification of distinct neural projections from retrograde tracing.

ABSTRACT: Comprehensive quantification of neuronal architectures underlying anatomical brain connectivity remains challenging. We introduce a method to identify the distinct axonal projection patterns from a source to a set of target regions and the count of neurons with each pattern. For a source region projecting to n targets, there are 2ⁿ - 1 theoretically possible projection types, although only a subset of these types typically exists. By injecting uniquely labeled retrograde tracers in k regions (k < n), one can experimentally count the cells expressing different combinations of colors in the source region^1,2. Such an experiment can be performed for n choose k combinations. The counts of cells with different color combinati

SUBMITTER: Venkadesh S 

PROVIDER: S-EPMC9882684 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature