Differential effects of WRAP53 transcript variants on non-small cell lung cancer cell behaviors.

ABSTRACT:

Background

The WD40-encoding RNA antisense to p53 (WRAP53) is an antisense gene of TP53 with three transcriptional start sites producing three transcript variants involved in the progression of non-small cell lung cancer. However, the mechanism by which these different transcript variants regulate non-small cell lung cancer cell behaviors is to be elucidated.

Methods

Two non-small cell lung cancer cell lines, A549 cells with wild-type p53 and H1975 with mutated p53, were transfected with WRAP53-1α and WRAP53-1β siRNA. The biological effects were assessed via colony formation, cell viability, apoptosis, cell cycle, wound healing and cell invasion assays, as well as immunoblotting.

Results

Knockdown of WRAP53-1α increased the mRNA and protein levels of p53; suppressed c

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC9882892 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature