Hepatitis C virus replication requires integrity of mitochondria-associated ER membranes.

ABSTRACT:

Background & aims

Chronic HCV infection causes cellular stress, fibrosis and predisposes to hepatocarcinogenesis. Mitochondria play key roles in orchestrating stress responses by regulating bioenergetics, inflammation and apoptosis. To better understand the role of mitochondria in the viral life cycle and disease progression of chronic hepatitis C, we studied morphological and functional mitochondrial alterations induced by HCV using productively infected hepatoma cells and patient livers.

Methods

Biochemical and imaging assays were used to assess localization of cellular and viral proteins and mitochondrial functions in cell cultures and liver biopsies. Cyclophilin D (CypD) knockout was performed using CRISPR/Cas9 technology. Viral replication was quantified by quantitative

SUBMITTER: Duponchel S 

PROVIDER: S-EPMC9883273 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature