Profiling of peripheral blood B-cell transcriptome in children who developed coeliac disease in a prospective study.

ABSTRACT:

Background

In coeliac disease (CoD), the role of B-cells has mainly been considered to be production of antibodies. The functional role of B-cells has not been analysed extensively in CoD.

Methods

We conducted a study to characterize gene expression in B-cells from children developing CoD early in life using samples collected before and at the diagnosis of the disease. Blood samples were collected from children at risk at 12, 18, 24 and 36 months of age. RNA from peripheral blood CD19⁺ cells was sequenced and differential gene expression was analysed using R package Limma.

Findings

Overall, we found one gene, HNRNPL, modestly downregulated in all patients (logFC -0·7; q = 0·09), and several others downregulated in those diagnosed with CoD already by

SUBMITTER: Oras A 

PROVIDER: S-EPMC9883278 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature