Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.

ABSTRACT: Chlorella sp. TLD 6B, a microalgae growing in the Taklamakan Desert, Xinjiang of China, is a good model material for studying the physiological and environmental adaptation mechanisms of plants in their arid habitats, as its adaptation to the harsh desert environment has led to its strong resistance. However, when using real-time quantitative polymerase chain reaction (RT-qPCR) to analyze the gene expression of this algae under abiotic stress, it is essential to find the suitable endogenous reference genes so to obtain reliable results. This study assessed the expression stability of 9 endogenous reference genes of Chlorella sp. TLD 6B under four abiotic stresses (drought, salt, cold and heat). These genes were selected based on the analysis results calculated by the three algorithmic proc

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC9883494 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature