Microraft arrays for serial-killer CD19 chimeric antigen receptor T cells and single cell isolation.

ABSTRACT: Chimeric antigen receptor T (CAR-T) cell immunotherapies have seen success in treating hematological malignancies in recent years; however, the results can be highly variable. Single cell heterogeneity plays a key role in the variable efficacy of CAR-T cell treatments yet is largely unexplored. A major challenge is to understand the killing behavior and phenotype of individual CAR-T cells, which are able to serially kill targets. Thus, a platform capable of measuring time-dependent CAR-T cell mediated killing and then isolating single cells for downstream assays would be invaluable in characterizing CAR-T cells. An automated microraft array platform was designed to track CD19 CAR-T cell killing of CD19+ target cells and CAR-T cell motility over time followed by CAR-T cell collection based

SUBMITTER: LaBelle CA 

PROVIDER: S-EPMC9883594 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature