B.infantis enhances immunotherapy for Guillain-Barre syndrome through regulating PD-1.

ABSTRACT:

Background

Guillain-Barré syndrome (GBS) is a rare, autoimmune disease. B.infantis is reported to be effective in alleviating GBS by regulating abnormal function of T helper (Th) cells.

Objectives

In this study, T cells were isolated from healthy and GBS patients. The therapeutic effect of Bifidobacterium infantis (B.infantis) and whether it is achieved by PD-1 was examined at cellular and animal models.

Methods

We used CCK-8, flow cytometry and real-time PCR to determine the differentiation of T cell subsets at cellular level. Then, an experimental autoimmune neuritis (EAN) animal model using six-week SD rats (n = 30, male) weighing 180-200 g was established to support the role of B. infantis in GBS through PD-1.

Results

B. infantis inhibited the proliferation

SUBMITTER: Shi P 

PROVIDER: S-EPMC9883859 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature