Revisiting chloroplast genomic landscape and annotation towards comparative chloroplast genomes of Rhamnaceae.

ABSTRACT:

Background

Massive parallel sequencing technologies have enabled the elucidation of plant phylogenetic relationships from chloroplast genomes at a high pace. These include members of the family Rhamnaceae. The current Rhamnaceae phylogenetic tree is from 13 out of 24 Rhamnaceae chloroplast genomes, and only one chloroplast genome of the genus Ventilago is available. Hence, the phylogenetic relationships in Rhamnaceae remain incomplete, and more representative species are needed.

Results

The complete chloroplast genome of Ventilago harmandiana Pierre was outlined using a hybrid assembly of long- and short-read technologies. The accuracy and validity of the final genome were confirmed with PCR amplifications and investigation of coverage depth. Sanger sequencing was used to co

SUBMITTER: Wanichthanarak K 

PROVIDER: S-EPMC9883906 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature