Histone methyltransferase SETD1A interacts with notch and promotes notch transactivation to augment ovarian cancer development.

ABSTRACT:

Background

High expression of SETD1A, a histone methyltransferase that specifically methylates H3K4, acted as a key oncogene in several human cancers. However, the function and underlying molecular mechanism of SETD1A in ovarian cancer (OV) remain markedly unknown.

Methods

The expression of SETD1A in OV were detected by Western blot and analyzed online, and the prognosis of STED1A in OV were analyzed online. The protein and mRNA levels were determined by Western blot and RT-qPCR. The cell proliferatin, migration and invasion were measured by CCK-8 and transwell assays. The protein interaction was detected by co-IP assay. The interaction between protein and DNA was performed by ChIP assay. The tumor growth in vivo was performed by xenograft tumor model.

Results

SETD1A

SUBMITTER: Chai H 

PROVIDER: S-EPMC9883963 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature