Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants.

ABSTRACT: Increasing crop yields through plant breeding is time consuming and laborious, with the generation of novel combinations of alleles being limited by chromosomal linkage blocks and linkage-drag. Meiotic recombination is essential to create novel genetic variation via the reshuffling of parental alleles. The exchange of genetic information between homologous chromosomes occurs at crossover (CO) sites but CO frequency is often low and unevenly distributed. This bias creates the problem of linkage-drag in recombination 'cold' regions, where undesirable variation remains linked to useful traits. In plants, programmed meiosis-specific DNA double-strand breaks, catalysed by the SPO11 complex, initiate the recombination pathway, although only ~5% result in the formation of COs. To study the role o

SUBMITTER: Hyde L 

PROVIDER: S-EPMC9884015 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature