MPK6-mediated HY5 phosphorylation regulates light-induced anthocyanin accumulation in apple fruit.

ABSTRACT: Light is known to regulate anthocyanin pigment biosynthesis in plants on several levels, but the significance of protein phosphorylation in light-induced anthocyanin accumulation needs further investigation. In this study, we investigated the dynamics of the apple fruit phosphoproteome in response to light, using high-performance liquid chromatography-tandem mass spectrometry analysis. Among the differentially phosphorylated proteins, the bZIP (basic leucine zipper) transcription factor, HY5, which has been identified as an anthocyanin regulator, was rapidly activated by light treatment of the fruit. We hypothesized that phosphorylated MdHY5 may play a role in light-induced anthocyanin accumulation of apple fruit. Protein interaction and phosphorylation assays showed that mitogen-activated

SUBMITTER: Xing Y 

PROVIDER: S-EPMC9884024 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature