Comparison of urine proteomes from tumor-bearing mice with those from tumor-resected mice.

ABSTRACT:

Objective

This study aimed to address on the most important concern of surgeons-whether to completely resect tumor. Urine can indicate early changes associated with physiological or pathophysiological processes. Based on these ideas, we conducted experiments to explore changes in the urine proteome between tumor-bearing mice and tumor-resected mice.

Method

The tumor-bearing mouse model was established with MC38 mouse colon cancer cells, and the mice were divided into the control group, tumor-resected group, and tumor-bearing group. Urine was collected 7 and 30 days after tumor resection. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used to identify the urine proteome, which was analyzed for differentially expressed proteins and functional annota

SUBMITTER: Heng Z 

PROVIDER: S-EPMC9884041 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature