Expanding and improving nanobody repertoires using a yeast display method: Targeting SARS-CoV-2.

ABSTRACT: COVID-19, caused by the coronavirus SARS-CoV-2, represents a serious worldwide health issue, with continually emerging new variants challenging current therapeutics. One promising alternate therapeutic avenue is represented by nanobodies, small single-chain antibodies derived from camelids with numerous advantageous properties and the potential to neutralize the virus. For identification and characterization of a broad spectrum of anti-SARS-CoV-2 Spike nanobodies, we further optimized a yeast display method, leveraging a previously published mass spectrometry-based method, using B-cell complementary DNA from the same immunized animals as a source of V_HH sequences. Yeast display captured many of the sequences identified by the previous approach, as well as many additional sequenc

SUBMITTER: Cross FR 

PROVIDER: S-EPMC9884143 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature