Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics.

ABSTRACT: While thousands of environmental metagenomes have been mined for the presence of novel biosynthetic gene clusters, such computational predictions do not provide evidence of their in vivo biosynthetic functionality. Using fluorescent in situ enzyme assay targeting carrier proteins common to polyketide (PKS) and nonribosomal peptide synthetases (NRPS), we applied fluorescence-activated cell sorting to tunicate microbiome to enrich for microbes with active secondary metabolic capabilities. Single-cell genomics uncovered the genetic basis for a wide biosynthetic diversity in the enzyme-active cells and revealed a member of marine Oceanospirillales harboring a novel NRPS gene cluster with high similarity to phylogenetically distant marine and terrestrial bacteria. Interesti

SUBMITTER: Kim WE 

PROVIDER: S-EPMC9884146 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature