Immortalized murine tenocyte cells: a novel and innovative tool for tendon research.

ABSTRACT: Primary tenocytes rapidly undergo senescence and a phenotypic drift upon in vitro monolayer culture, which limits tendon research. The Ink4a/Arf locus encodes the proteins p16^Ink4a/Arf and p14^ARF (p19^ARF in mice) that regulate cell cycle progression and senescence. We here established an immortalized cell line using tenocytes isolated from Ink4a/Arf deficient mice (Ink4a/Arf^-/-). These cells were investigated at three distinct time points, at low (2-5), intermediate (14-17) and high (35-44) passages. Wild-type cells at low passage (2-5) served as controls. Ink4a/Arf^-/- tenocytes at all stages were comparable to wild-type cells regarding morphology, expression of tenogeneic genes (collagen type 1, 3 and 5, Scleraxis, Tenomodulin and Ten

SUBMITTER: Oreff GL 

PROVIDER: S-EPMC9884217 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature