Targeting epigenetic features in clear cell sarcomas based on patient-derived cell lines.

ABSTRACT:

Background

Clear cell sarcomas (CCSs) are translocated aggressive malignancies, most commonly affecting young adults with a high incidence of metastases and a poor prognosis. Research into the disease is more feasible when adequate models are available. By establishing CCS cell lines from a primary and metastatic lesion and isolating healthy fibroblasts from the same patient, the in vivo process is accurately reflected and aspects of clinical multistep carcinogenesis recapitulated.

Methods

Isolated tumor cells and normal healthy skin fibroblasts from the same patient were compared in terms of growth behavior and morphological characteristics using light and electron microscopy. Tumorigenicity potential was determined by soft agar colony formation assay and in vivo xenograft

SUBMITTER: Karner C 

PROVIDER: S-EPMC9884415 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature