Enrichment and fragmentation approaches for enhanced detection and characterization of endogenous glycosylated neuropeptides.

ABSTRACT: Glycosylated neuropeptides were recently discovered in crustaceans, a model organism with a well-characterized neuroendocrine system. Several workflows exist to characterize enzymatically digested peptides; however, the unique properties of endogenous neuropeptides require methods to be re-evaluated. We investigate the use of hydrophilic interaction liquid chromatography (HILIC) enrichment and different fragmentation methods to further probe the expression of glycosylated neuropeptides in Callinectes sapidus. During the evaluation of HILIC, we observed the necessity of a less aqueous solvent for endogenous peptide samples. This modification enabled the number of detected neuropeptide glycoforms to increase almost two-fold, from 18 to 36. Product ion-triggered electron-transfer/higher-energ

SUBMITTER: Phetsanthad A 

PROVIDER: S-EPMC9884999 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature