Engineering a mevalonate pathway in Halomonas bluephagenesis for the production of lycopene.

ABSTRACT:

Introduction

Red-colored lycopene has received remarkable attention in medicine because of its antioxidant properties for reducing the risks of many human cancers. However, the extraction of lycopene from natural hosts is limited. Moreover, the chemically synthesized lycopene raises safety concerns due to residual chemical reagents. Halomonas bluephagenesis is a versatile chassis for the production of fine chemicals because of its open growth property without sterilization.

Methods

A heterologous mevalonate (MVA) pathway was introduced into H. bluephagenesis strain TD1.0 to engineer a bacterial host for lycopene production. A pTer7 plasmid mediating the expression of six MVA pathway genes under the control of a phage P_Mmp1 and an Escherichi

SUBMITTER: Su Q 

PROVIDER: S-EPMC9885113 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature