Mas-related G protein-coupled receptor MRGPRX2 in human basophils: Expression and functional studies.

ABSTRACT:

Background

Occupancy of MRGPRX2 heralds a new era in our understandings of immediate drug hypersensitivity reactions (IDHRs), but a constitutive expression of this receptor by basophils is debated.

Objective

To explore the expression and functionality of MRGPRX2 in and on basophils.

Methods

Basophils from patients with birch pollen allergy, IDHRs to moxifloxacin, and healthy controls were studied in different conditions, that is, in rest, after stimulation with anti-IgE, recombinant major birch pollen allergen (rBet v 1), moxifloxacin, fMLP, substance P (SP), or other potential basophil secretagogues. In a separate set of experiments, basophils were studied after purification and resuspension in different media.

Results

Resting whole blood basophils barely expr

SUBMITTER: Toscano A 

PROVIDER: S-EPMC9885256 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature