New insights on the interaction between m⁶A modification and non-coding RNA in cervical squamous cell carcinoma.

ABSTRACT:

Background

N⁶-Methyladenosine (m⁶A) and long non-coding RNAs (lncRNAs) are both crucial regulators in human cancer growth and metastasis. However, their regulation on cervical squamous cell carcinoma (CSCC) is largely unclear. The present study aimed to explore the role of m⁶A-associated lncRNAs in CSCC.

Methods

We screened the expression of methylation modification-related enzymes in CECC samples from TCGA. The qRT-PCR was used to detect METTL3 and lncRNA METTL4-2 expression. The biological activities of METTL3 in CSCC cells were evaluated by CCK-8, colony formation, transwell, wound healing, and xenograft tumor assays, respectively. The SRAMP tool was used to screen m⁶A modification sites of METTL4-2. Finally, the quantitative an

SUBMITTER: Shen G 

PROVIDER: S-EPMC9885588 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature