Establishment and characterization of a HER2-enriched canine mammary cancerous myoepithelial cell line.

ABSTRACT:

Background

Canine mammary tumors (CMTs) have a poor prognosis, along with tumor recurrence and metastasis. Cell lines are vital in vitro models for CMT research. Many CMT epithelial cell lines were reported. However, canine mammary myoepithelial cells, the contractile component of the canine mammary tissue were overlooked. This study aimed at establishing such a cell line. CMT-1 cell line was obtained from a canine mammary tumor CMT-1 and characterized molecularly through qPCR, western blotting, immunochemistry and immunofluorescence. Its doubling time, cytogenetic analysis and migration rate were evaluated using growth study, karyotype analysis and wound healing assay respectively. To determine its tumorigenesis, xenograft transplantation was performed.

Results

CMT-1 tumor

SUBMITTER: Chen A 

PROVIDER: S-EPMC9885638 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature