Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep.

ABSTRACT: Environmental effects on gene expression and offspring development can be mediated by epigenetic modifications. It is well established that maternal diet influences DNA methylation patterns and phenotypes in the offspring; however, the epigenetic effects of paternal diet on developing offspring warrants further investigation. Here, we examined how a prepubertal methionine-enriched paternal diet affected sperm DNA methylation and its subsequent effects on embryo gene expression. Three treatment and three control rams were bred to seven ewes, and blastocysts were flushed for RNA extraction. Semen was collected from all rams and submitted for reduced representation bisulfite sequencing analysis. In total, 166 differentially methylated cytosines were identified in the sperm from treatment vers

SUBMITTER: Townsend J 

PROVIDER: S-EPMC9885981 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature